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Differential expression of functional cannabinoid receptors in human bladder detrusor and urothelium.

By June 27th, 2019 Urinary Bladder Diseases

Authors:

Vikas Tyagi, Brian J. Philips, Ruthie Su, Marc C. Smaldone, Vickie L. Erickson, Michael B. Chancellor, Naoki Yoshimura, Pradeep Tyagi


Published in The Journal of Urology

April 2009

 

Abstract

PURPOSE:
Although cannabinoid receptor expression has been demonstrated in human brain and other peripheral neuronal tissues, definitive expression of these receptors in the human bladder has not been reported. Consequently we investigated the expression of functional cannabinoid 1 and 2 receptors in human bladder detrusor and urothelium.

MATERIALS AND METHODS:
Human bladders were micro-dissected for detrusor (6) and urothelium (8), and analyzed for cannabinoid 1 and 2 mRNA expression using real-time quantitative polymerase chain reaction, and for protein expression using immunohistochemistry and Western blot. Functional response of these receptors was tested by studying the effect of selective cannabinoid 1 and 2 agonists on nerve evoked smooth muscle contraction.

RESULTS:
Quantitative polymerase chain reaction analysis revealed differential expression of cannabinoid 1 and 2 receptors in detrusor and urothelium. The expression of cannabinoid 1 and 2 receptor mRNA in urothelium was approximately 2-fold higher than in detrusor, although this was not significant (p >0.05). Cannabinoid 1 receptor mRNA expression was significantly higher than cannabinoid 2 receptor expression in the 2 tissue subtypes (p

 

DOI: 10.1016/j.juro.2008.11.078

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Citation:

Tyagi V, Philips BJ, Su R, et al. Differential Expression of Functional Cannabinoid Receptors in Human Bladder Detrusor and Urothelium. J Urol. 2009;181(4):1932-1938. doi:10.1016/j.juro.2008.11.078